DNA Nanomachines Investigated By Non-Denaturing Mass Spectrometry

Interactions of Anticancer Therapeutics with DNA Investigated via Mass Spectrometry APPROVED BY SUPERVISING COMMITTEE:

Natural Waxes Investigated by Soft Ionization Mass Spectrometry*

Field ionization (FI) and field desorption (FD) mass spectra of 35 aliphatic long-chain and branched long-chain compounds, representing the m ajor six classes of constituents of natural waxes, are examined. In the FI mode the molecular ions are usually formed at comparatively low levels, while in FD such species are almost exclusively generated. In addition, some fast atom bom bardm ent mass sp...

DNA sequence analysis by MALDI mass spectrometry.

Conventional DNA sequencing is based on gel electrophoretic separation of the sequencing products. Gel casting and electrophoresis are the time limiting steps, and the gel separation is occasionally imperfect due to aberrant mobility of certain fragments, leading to erroneous sequence determination. Furthermore, illegitimately terminated products frequently cannot be distinguished from correctl...

Non-enzymatic posttranslational modifications of bovine serum albumin by oxo-compounds investigated by high-performance liquid chromatography-mass spectrometry and capillary zone electrophoresis-mass spectrometry.

Non-enzymatic posttranslational modifications of bovine serum albumin (BSA) by various oxo-compounds (glucose, ribose, glyoxal and glutardialdehyde) have been investigated using high-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE). Both of these methods used mass spectrometric (MS) detection. Three enzymes (trypsin, pepsin, proteinase K) were used to digest gly...

Surface-immobilized DNA nanomachines

We are working towards the construction of hybrid bioelectronic systems in which DNA nanomachines are integrated with semiconductor circuitry, where DNA performs the function of highly parallel, low-power processing of biomolecular signals, and silicon is used for high-speed data processing and control. This technology has applications in computation and smart biosensing, and surface immobiliza...